Visualization of trans-interactions of a protocadherin-α between processes originating from single neurons

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V-α4ΔICD α4ΔICD-EC4-V tended show slightly smaller than significant differences confirmed 1E). results indicate significantly affect By replacing ddFP-B V-α4ΔICD, respectively, ddGFP-A-inserted (α4ΔICD-EC4-GA) ddFP-B-inserted (B-α4ΔICD) 2A). variants affinities ddGFP-A, ddFP-B1 ddFP-B3 (Kd = 3 μM 40 μM, respectively).45Ding combinations α4ΔICD-EC4-GA/B1-α4ΔICD α4ΔICD-EC4-GA/B3-α4ΔICD designated sIPAD (separate type Indicator Protocadherin-Alpha Dimerization)-1 sIPAD-3, investigate detectability sIPADs co-cultured HEK293T components. EBFP2- γB2ΔEC1ΔICD (γB2ΔEC1ΔICD-EB γB2ΔEC1ΔICD-mCh) co-expressed α4ΔICD-EC4-GA B-α4ΔICD, observed mCherry-positive confirming 2B). intensity comparable sIPAD-1 sIPAD-3 2C). enhances address possibility, α4s 2D). so 2E). result suggests ddFP-Bs enhance interac